Role of Different Transporting Systems in the Secretion of Alkaloids by Hairy Roots of Catharanthus roseus (L) G. Don
نویسندگان
چکیده
Knowledge on the biosynthetic pathways of the monoterpene alkaloids is enormous, but little is known about their mechanism of transporting system from the plant cell. There is not concrete evidence confirming the role of ABC transporters in the secretion of monoterpene indole alkaloids (MIAs) in Catharanthus roseus. Therefore, in order to determine the role of different transporting systems involved in the MIAs translocation, we employed a pharmacological approach by using transport inhibitors such as, KCN, Na3VO4, quinidine and glibenclamide in hairy root cultures of C. roseus. It was found that the accumulation of ATP drastically decreased in the presence of KCN or 100 μM acetylsalicylic acid (ASA)/100 μM KCN. The treatment with the inhibitors KCN and glibenclamide in the presence of ASA significantly increased the ajmalicine secretion compared to the control. The secretion of serpentine was undetected during the first 24 h in all the samples. Treatment with the inhibitors quinidine and glibenclamide provoked a significant reduction of serpentine secretion in the hairy roots compared to the control. Based on our results, we found evidence that ABC transporters might participate in the secretion of MIAs by C. roseus hairy roots.
منابع مشابه
Potent Antioxidant Properties of rolB-transformed Catharanthus roseus (L.) G. Don
Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of r...
متن کاملPotent Antioxidant Properties of rolB-transformed Catharanthus roseus (L.) G. Don
Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of r...
متن کاملRapid screening of antioxidant activity, fracture rate and scavenging of free radicals by hairy root of Periwinkle (Catharanthus roseus L. G. Don)
Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rol...
متن کاملRapid screening of antioxidant activity, fracture rate and scavenging of free radicals by hairy root of Periwinkle (Catharanthus roseus L. G. Don)
Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rol...
متن کاملبررسی اثر کادمیوم و نیکل بر الگوی تولید آلکالوئیدها در گیاه پروانش (Catharanthus roseus (L.) G. Don)
Tropical plant, Catharanthus roseus contains worthy alkaloids such as vinblastine and ajmalicine. Since amount and pattern of alkaloid production could be affected by different environmental conditions, the effect of two elements of cadmium and nickel were investigated. Plants in the greenhouse conditions using a chemically inert substrate were cultivated and in a complete randomized block desi...
متن کامل